Description
Clinical Description
Nucleofill 20, now commonly referred to as Nucleofill Medium, is a polynucleotide-based biostimulating injectable designed for professional aesthetic medicine applications. Developed by Promoitalia Medical Aesthetics, the product contains highly purified polynucleotides at a concentration of 20 mg/ml (2%), formulated to support skin revitalisation, hydration, and tissue quality improvement.
The product works through several complementary mechanisms. Polynucleotides help promote fibroblast activity, support extracellular matrix function, provide antioxidant protection against free radicals, and contribute to long-lasting hydration through water-binding properties. These actions are intended to improve skin elasticity, firmness, and overall skin quality while addressing signs associated with environmental stress and skin ageing.
Nucleofill Medium is typically used in patients presenting with dehydrated skin, mild-to-moderate wrinkles, loss of elasticity, oxidative stress-related skin changes, stretch marks, and superficial scars. It is supplied as a sterile injectable medical device for use exclusively by qualified healthcare professionals.
What Nucleofill 20 – Nucleofill Medium Is Similar To
Nucleofill Medium belongs to the same category as other injectable skin quality products and biostimulators such as Profhilo®, Plenhyage®, and other polynucleotide-based regenerative treatments.
Compared with Profhilo®, which relies on stabilised hyaluronic acid for tissue hydration and remodelling, Nucleofill Medium uses purified polynucleotides as its primary active component. Both products aim to improve skin quality and hydration, but their biological mechanisms differ.
Compared with stronger polynucleotide formulations such as Nucleofill Strong, Nucleofill Medium contains a lower concentration and viscosity profile intended for patients with mild-to-moderate signs of ageing or dehydration.
Nucleofill Medium is also frequently compared with other regenerative injectables used around delicate facial areas, including protocols involving Nucleofill 20 eyes, although treatment selection should always be based on clinical assessment and manufacturer guidance.
This comparison is provided for clinical orientation only and does not imply interchangeability.
Treatment Areas / Indications
According to publicly available manufacturer and distributor documentation, Nucleofill Medium may be used for:
- Face
- Neck
- Décolletage
- Hands
- Abdomen
- Thighs
- Areas affected by stretch marks
- Areas affected by superficial scars
- Skin exposed to oxidative stress
- Dehydrated skin
- Mild-to-moderate skin ageing concerns
Treatment suitability should always be determined by a qualified healthcare professional.
Usage / Administration (IFU-Only Section)
Injection Depth
Deep dermis bio-restructuring is referenced in publicly available manufacturer materials.
Volume per Injection Site
Not provided by manufacturer in publicly accessible documentation.
Needle Gauge
The product is supplied with:
- 2 × 30G needles
- Needle length approximately 12–13 mm
Frequency / Number of Sessions
Not provided by manufacturer in publicly available IFU documentation.
Some practitioners utilise multi-session protocols and maintenance treatments; however, these approaches are not included in the manufacturer’s publicly available official IFU guidance.
Professional Use Statement
Nucleofill Medium should be administered only by qualified healthcare professionals trained in injectable aesthetic procedures and under appropriate aseptic conditions.
Ingredient List
Active Ingredients
- Polynucleotide gel 2% (20 mg/ml)
Product Characteristics
- Molecular weight approximately 2,500 kDa
- pH: 6.8–7.2
- Viscosity: 28–43 Pa·s
Excipients
Not provided by manufacturer in publicly available documentation.
Hyaluronic Acid Concentration
Not applicable.
Lidocaine
No lidocaine reported in publicly available manufacturer information.
Presentation & Longevity
Packaging
Nucleofill Medium is supplied as:
- 1 × 1.5 ml sterile syringe or vial (depending on market presentation)
- 2 × 30G needles included in the package
Shelf Life
Not provided by manufacturer in publicly available documentation.
Treatment Duration / Longevity
The duration of results is not provided by manufacturer in publicly available IFU documentation.
Clinical outcomes may vary according to skin condition, treatment protocol, patient characteristics, and practitioner assessment.
Safety Notes / Contraindications
Publicly available product documentation provides limited contraindication information. Standard precautions include:
- Use only by trained healthcare professionals.
- Do not use in patients with known hypersensitivity to any product component.
- Do not inject into areas affected by active infection, inflammation, or skin disease.
- Product safety during pregnancy and breastfeeding has not been established in publicly available documentation.
- Follow all manufacturer instructions and local regulatory requirements.
Regulatory Status
- CE-marked medical device.
- Classified as a Class III medical device according to publicly available distributor and manufacturer information.
- Professional-use product intended for qualified healthcare practitioners.
UKCA status: verify via current IFU or authorised distributor documentation.
FAQ
1. What is Nucleofill 20 Medium?
Nucleofill 20 Medium is a polynucleotide-based injectable biostimulator designed to improve skin quality through hydration, antioxidant activity, and support of fibroblast function. The product contains 20 mg/ml purified polynucleotides and is intended for professional aesthetic use.
2. How does Nucleofill Medium work?
The polynucleotides contained within Nucleofill Medium help support extracellular matrix function, bind water molecules, and stimulate cellular processes associated with collagen and elastin production. The formulation also demonstrates antioxidant activity that may help protect tissues from oxidative stress.
3. Is Nucleofill 20 suitable for the eye area?
Many aesthetic practitioners utilise polynucleotide treatments around delicate facial areas, and searches for Nucleofill 20 eyes are common. However, treatment suitability depends on clinical assessment, practitioner expertise, and official product guidance. Healthcare professionals should always follow manufacturer recommendations and applicable training protocols.
4. How long do Nucleofill Medium results last?
The manufacturer does not specify a definitive duration of effect in publicly available documentation. Clinical longevity varies depending on patient characteristics, treatment area, skin condition, and treatment protocol. Maintenance schedules should be determined by the treating practitioner.
5. How does Nucleofill Medium compare with hyaluronic acid skin boosters?
Unlike hyaluronic acid-based skin boosters, Nucleofill Medium relies on purified polynucleotides. While both categories aim to improve hydration and skin quality, polynucleotides focus primarily on biostimulation, antioxidant activity, and regenerative support rather than HA-mediated tissue hydration alone.
